Human Synthetic Antibody Library

Hucal platinum is a synthetic library of 45 billion fully human antibodies in fab format.

Human synthetic antibody library.

Construction of a fully synthetic human scfv antibody library with cdr3 regions randomized by a split mix split method and its application chang cheng yin1 2 li li ren 2 lin lin zhu2 xiang bin wang zhong zhang hua liang huang2 and xi yun yan1 y 1national laboratory of biomacromolecules and cas ut joint laboratory of structural virology and. 19252839 indexed for medline mesh terms. It has been designed to cover the human antibody diversity to be flexible for further engineering and to yield antibodies with high expression titers. Amino acid sequence.

This report describes the design generation and testing of ylanthia a fully synthetic human fab antibody library with 1 3e 11 clones. Fully synthetic human combinatorial antibody libraries hucal based on modular consensus frameworks and cdrs randomized with trinucleotides j mol biol. We describe here how to construct a large human synthetic single chain fv scfv antibody library displayed on phages where in vivo formed complementarity determining regions cdrs are shuffled combinatorially onto germline derived human variable region frameworks. Presented here are the design construction and application of ylanthia a fully synthetic human fab antibody phage display library built on 36 fixed germline vh vl framework pairs selected for their favorable biophysical properties.

Antibody phage display was later used by carlos f. The hucal platinum phage library contains more than 45 billion functional human antibody genes in a fab format. J mol biol 340 5 1073 1093. Ylanthia comprises 36 fixed immunoglobulin ig variable heavy vh variable light vl chain pairs which cover a broad range of canonical complementarity determin.

With this goal in mind an antibody library that is optimized for processability has been generated. Precise control over design enables the use of highly optimized human frameworks and the introduction of defined chemical diversity at positions that are most likely to contribute to antigen recognition. Lee cv liang wc dennis ms eigenbrot c sidhu ss fuh g 2004 high affinity human antibodies from phage displayed synthetic fab libraries with a single framework scaffold.